Figure 1.

Yeast two-hybrid interaction between GADD34 and Lyn. (Upper) Block diagram of Lyn's domains. Unique, SH3, SH2, and catalytic domains as well as the portion of Lyn used as bait are shown. (Lower) YRG-2 yeast transformants harboring pGBT9-Lyn, the human bone marrow library GADD34 plasmid, and control plasmids p53 and pSV40 as indicated were streaked on a plate lacking leucine, tryptophan, and histidine. The plate was incubated at 30°C for 3 days. Histidine-independent growth indicates the two-hybrid interaction between plasmid-encoded proteins.