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. 2017 Oct 26;11(10):e0006025. doi: 10.1371/journal.pntd.0006025

Fig 2. Shift in pH, temperature and L-arginine availability influences 5.1 aap3 transcripts amount.

Fig 2

The 5.1 and 4.7 aap3 mRNA levels were based on quantification of the target and were normalized by gapdh expression. (A) Copy number of 5.1 aap3 transcripts determined after starvation or supplementation with 400 μM L-arginine at 25 and 34°C, and in pH 5.0 or pH 7.0. (a) p < 0.05, comparing the control and the changed conditions of starvation (-) or L-arginine supplementation (+) at 25°C or 34°C and in pH 5.0 or pH 7.0. (b) p < 0.05, comparing the changed conditions of starvation or L-arginine supplementation. (c) p < 0.05, comparing the changed conditions at 25°C or 34°C in pH 5.0 and pH 7.0. (d) p < 0.05, comparing the changed conditions at 25°C and 34°C in pH 5.0 or pH 7.0. (B) Copy number of 4.7 aap3 transcripts determined after starvation or supplementation with 400 μM L-arginine at 25 and 34°C, and in pH 5.0 and pH 7.0. The values are the mean ± SEM of three independent biological replicates. (C) Control parasites were collected before amino acid starvation and/or L-arginine supplementation.