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. 2017 Nov 16;14:223. doi: 10.1186/s12974-017-0994-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Repeated low-dose LPS intraperitoneal injection preconditioning did not cause inflammation in the SN. We investigated the levels of pro-inflammatory cytokines and the expression of Iba-1 in the right SN of rats at 1, 7, 14, and 28 days post to the peripheral LPS injection. There was no significant difference between the control group and any other time points of the LPS (i.p.) group in the amounts of TNF-α and IL-1β nor in the expression of Iba-1. a, b The levels of TNF-α (a) and IL-1β (b) in the right SN were detected by ELISA. c, d The expression of Iba-1 in the right SN was quantified by measuring band intensities using ImageJ software. The values were normalized to GAPDH. The data are presented as the mean ± SEM (n = 6)