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. 2017 Nov 17;16:173. doi: 10.1186/s12943-017-0741-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overview of 3C-based C-techniques. After crosslinking, chromatin is digested into 4-10 kb pieces by restriction enzymes, followed by ligation and reverse crosslinking to change the crosses into lines. An additional digestion step is added in 4C. LMA is used instead of PCR, which is commonly used in 5C; biotinylation and streptavidin are used in Hi-C, and a chromatin immunoprecipitation (CHIP) step is added in ChIA-PET