Table 2.

Primers used for validation of microarray analysis by RT-qPCR

Genes	Primer sequence (5′-3′)	Amplicon	Ta	Accession number	Reference
cpn2	F: AAGGATGGAGAGCTGCTGTT	154 bp	59 °C	XM_014180427.1	New design
	R: CTATCCCTCCGGTGAAGTCC
gsta3	F: ACCTCCCTGTGTTCGAGAAG	231 bp	59 °C	NM_001140755.1	New design
	R: CGTCATCAGGTTGAGGCTTC
hspa4	F: CTACGCTGTGGAAATCGTGG	242 bp	59 °C	XM_014136794.1	New design
	R: CACTTAACCCCTCCTCTGCA
hspa5	F: CTACGCCTACTCGCTCAAGA	166 bp	59 °C	XM_014136127.1	New design
	R: CTCCTTCTTCTTGGCCTGGA
tryp	F: GATACATGGACGGAGGCAGA	210 bp	59 °C	NM_001140895.2	New design
	R: ACAGGGAGGAAAGCAGCTAG
elovl5b	F: ACAAAAAGCCATGTTTATCTGAAAGA	141 bp	60 °C	NM_001136552.1	Betancor et al. [63]
	R: AAGTGGGTCTCTCTGGGGCTGTG
elovl6	F: ATCTGAGGAAACCGCTGGTG	177 bp	56 °C	XM_014199191.1	New design
	R: CAAAGGCGTAGGCCCAAAAC
fads2d6a	F: GCTGGCCCATCTAGCAGAAA	119 bp	59 °C	NM_001123575.2	New design
	R: TGTCTGAGCCAAGTCACACC
βactin	F: ATCCTGACAGAGCGCGGTTACAGT	112 bp	60 °C	AF012125	McStay et al. [64]
	R: TGCCCATCTCCTGCTCAAAGTCCA
ef1a	F: CACCACCGGCCATCTGATCTACAA	78 bp	60 °C	DQ834870	Ytteborg et al. [65]
	R: TCAGCAGCCTCCTTCTCGAACTTC
rpl1	F: ACTATGGCTGTCGAGAAGGTGCT	120 bp	60 °C	NM_001140826.1	Carmona-Antoñanzas et al. [66]
	R: TGTACTCGAACAGTCGTGGGTCA

cpn2 calpain-2, gsta3 glutathione S-transferase alpha 3, hspa4 heat shock protein 4-like, hspa5 heat shock protein 5-like, tryp trypsin, elovl5b fatty acyl elongase 5 isoform b, elovl6 fatty acyl elongase 6, fads2d6a delta-6 fatty acyl desaturase isoform a, βactin β-actin, ef1a elongation factor 1 alpha, rpl1 ribosomic protein L1