Table 3.
Validation of microarray analysis by RT-qPCR
| Genes | FC Diploids V/M | FC Triploids V/M | ||||
|---|---|---|---|---|---|---|
| Microarray | RT-qPCR | p-value | Microarray | RT-qPCR | p-value | |
| Calpain2 (cpn2) | +2.76 | +1.78 | >0.05 | +1.44 | −1.34 | >0.05 |
| Glutathione S-transferase alpha 3 (gsta3) | +1.27 | +1.19 | >0.05 | +1.99 | +1.74* | 0.012 |
| Heat shock protein 4 (hspa4) | −1.68 | −1.45 | >0.05 | −1.47 | −1.35 | >0.05 |
| Heat shock protein 5 (hspa5) | −1.68 | −1.44 | >0.05 | −2.49 | −2.69 | >0.05 |
| Trypsin (tryp) | +4.32 | +3.46 | >0.05 | +2.39 | +1.80 | >0.05 |
| Fatty acyl elongase 5 isoform b (elovl5b) | +1.84 | +1.53 | >0.05 | +1.71 | +1.23 | >0.05 |
| Fatty acyl elongase 6 (elovl6) | +1.96 | +1.29 | >0.05 | +2.70 | +1.73 | >0.05 |
| Delta-6 fatty acyl desaturase isoform a (fads2d6a) | +1.15 | +1.98 | >0.05 | +1.29 | −1.22 | >0.05 |
Data are presented as the fold change (FC) between expression levels in diploid and triploid salmon fed the vegetable (V) diet versus the marine (M) diet. Asterisks indicate fold changes that are statistically significant (Mann-Whitney test, p < 0.05)