Taurine transporter protein and taurine content in tibialis anterior muscles from 28 d and 70 d wild-type, mdx and mdx taurine supplemented mice.

A. Western blot of taurine transporter protein (TauT, top) and Stain Free gel showing total protein (bottom) from tibialis anterior (TA) 28 and 70 d wild-type (WT), mdx and mdx taurine supplemented (mdx tau) mice. The four point calibration curve is indicated. Two different molecular weight markers are shown between the samples and the calibration curve, with the protein sizes indicated. B. Densities of total protein (left) and TauT protein (right) plotted against the calibration curve volumes loaded. The linear regression, y = mx + c is shown, along with correlation coefficient (r²). C. The TauT protein abundance is shown for 28 d (black bars) and 70 d (white bars) WT, mdx and mdx tau mice. D. TA taurine content represented as in C. Lines above specific bars indicate significant difference between groups (p<0.05). One way ANOVA with Sidak’s post-hoc analyses. Data presented as means + SD with n indicated in respective bars.