Skip to main content
. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Cell Biochem Biophys. 2017 Jun 29;75(3-4):335–349. doi: 10.1007/s12013-017-0813-6

Figure 15.

Figure 15

Monitoring NADPH oxidase-2 activity in dHL60 cells using secondary assays. (A) Effect of SOD, catalase, DPI, and VAS2870 on the yield of 2-OH-E+ formed from HE probe by dHL60 cells stimulated with PMA, as measured by rapid HPLC analyses. (B) Effect of SOD, CAT, DPI, and VAS2870 on the time-dependent increase in fluorescence intensity due to oxidation of Amplex Red probe, induced by PMA. (This research was originally published in Journal of Biological Chemistry. Zielonka, J., Cheng, G., Zielonka, M., Ganesh, T., Sun, A., Joseph, J., Michalski, R., O’Brien, W. J., Lambeth, J. D., & Kalyanaraman, B. High-throughput assays for superoxide and hydrogen peroxide: design of a screening workflow to identify inhibitors of NADPH oxidases. J. Biol. Chem. 2014; 289: 16176–16189. © the American Society for Biochemistry and Molecular Biology.) (79)