EPR spin trapping of superoxide generated by NADPH oxidase. DEPMPO spin trap was incubated with dHL60 cells in the absence (control) or presence of PMA. Where indicated, SOD or catalase was also present. (This research was originally published in Journal of Biological Chemistry. Zielonka, J., Cheng, G., Zielonka, M., Ganesh, T., Sun, A., Joseph, J., Michalski, R., O’Brien, W. J., Lambeth, J. D., & Kalyanaraman, B. High-throughput assays for superoxide and hydrogen peroxide: design of a screening workflow to identify inhibitors of NADPH oxidases. J. Biol. Chem. 2014; 289: 16176–16189. © the American Society for Biochemistry and Molecular Biology.) (79)