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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Cell. 2017 Oct 12;171(5):1057–1071.e11. doi: 10.1016/j.cell.2017.09.029

Figure 1. Increased IL-1β production and control of L. monocytogenes intracellular growth in Ch25h−/− macrophages involves ASC-dependent inflammasome activation.

Figure 1

IL-1β enzyme-linked immunosorbent assay (ELISA) from supernatants (A, B) and bacterial colony forming units (CFU) from cell lysates (C, D) of control (EtOH) or 25-HC (1uM) treated Ch25h+/−Asc+/+ and Ch25h−/−Asc+/+ (A, C) or Ch25h+/−Asc−/− and Ch25h−/−Asc−/− (B, D) BMDMs after 24 hr of L. monocytogenes infection (M.O.I. 10:1). (E) IL-1β ELISA from supernatants of LPS and LPS+ATP stimulated BMDMs of the indicated genotypes. (n=3 per genotype, means+/−SD). *P<0.05, **P<0.01, ***P < 0.005 (unpaired Students t test). See also Figure S1.

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