REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fixable viability dye eFluor780	eBioscience	Cat# 65-0865-14
Anti-mouse IL-17A Monoclonal Antibody (eBio17B7), PE	eBioscience	Cat# 12-7177-81
Anti-mouse CD4 Monoclonal Antibody (RM4-5), APC	Tonbo Biosciences	Cat# 20-0042-U100
Anti-mouse TCRβ Monoclonal Antibody (H57-597), Pacific Blue	Biolegend	Cat# 109226
Fixable viability dye eFluor780	eBioscience	Cat# 65-0865-14
Anti-mouse LDLR Polyclonal Antibody, PE	Sino Biological	Cat# 50305-R032-P-25
Anti-mouse CD4 Monoclonal Antibody (RM4-5), APC	Tonbo Biosciences	Cat# 20-0042-U100
Anti-mouse TCRβ Monoclonal Antibody (H57-597), Pacific Blue	Biolegend	Cat# 109226
Anti-mouse F4/80 Monoclonal Antibody (BM8.1), APC	Tonbo Biosciences	Cat# 20-4801-U100
Anti-Phospho-S6 Ribosomal Protein (Ser240/244) Monoclonal Antibody (D68F8)	Cell Signaling Technologies	Cat# 5364S
Anti-Phospho-p70 S6 Kinase (Thr389) Monoclonal Antibody (108D2)	Cell Signaling Technologies	Cat# 9234S
Anti-p70 S6 Kinase Monoclonal Antibody (49D7)	Cell Signaling Technologies	Cat# 9234T
Anti-mouse Cytochrome C Monoclonal Antibody (6H2.4), Alexa488	Biolegend	Cat# 612308
Anti-mouse NLRP3/NALP3 Monoclonal Antibody (Cryo-2)	Adipogen	Cat# AG-20B-0014-C100
Anti-mouse Asc Polyclonal Antibody (AL177)	Adipogen	Cat# AG-25B-0006-C100
Anti-mouse Caspase-1 Polyclonal Antibody (M-20)	Santa Cruz Biotechnology	Cat# sc-514
Anti-mouse Tomm20 Polyclonal Antibody	One World Lab	Cat# C16678
Anti-mouse α-Tubulin Monoclonal Antibody (DM1A), Biotin	eBioscience	Cat# 13-4502-80
Bacterial and Virus Strains
Listeria monocytogenes	A gift from D. Portnoy	N/A
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Recombinant murine IL-β	Peprotech	Cat# 211-11B
MitoSOX Red Mitochondrial Superoxide Indicator	ThermoFisher Scientific	Cat# M36008
MitoTracker Deep Red FM	ThermoFisher Scientific	Cat# M22426
MitoTracker Green FM	ThermoFisher Scientific	Cat# M7514
Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM)	ThermoFisher Scientific	Cat# T668
MitoTEMPO	Millipore Sigma	Cat# SML0737
DAPI, FluoroPure grade	ThermoFisher Scientific	Cat# D21490
ATP	InvivoGen	Cat# TLRL-ATP
Nigericin sodium salt	Millipore Sigma	Cat# N7143
Poly(deoxyadenylic-thymidylic)(dA:dT) acid sodium salt	Millipore Sigma	Cat# P0883-10UN
FlaTOX	von Moltke et al., 2012 (a gift from R. Vance)	N/A
Imject Alum Adjuvant	ThermoFisher Scientific	Cat# 77161
Lipopolysaccharide from E. coli O111:B4	Millipore Sigma	Cat# L4391
25-hydroxycholesterol	Avanti Polar Lipids	Cat# 700019P
Cholesterol-Water Soluble	Millipore Sigma	Cat# C4951
Methyl-β-cyclodextrin	Millipore Sigma	Cat# C4555
Rapamycin	LC Laboratories	Cat# R-5000
Cytochalasin D	Millipore Sigma	Cat# C8273
Filipin III from Streptomyces filipinensis	Millipore Sigma	Cat# F4767
Digitonin	Millipore Sigma	Cat# D141
Ethidium bromide	Millipore Sigma	Cat# E1510
2′,3′-Dideoxycytidine (ddC)	Millipore Sigma	Cat# D5782
GW3965 hydrochloride	Millipore Sigma	Cat# G6295
Lipoprotein, High Density	Lee Biosolutions	Cat# 361-10-0.01
Brain Heart Infusion Agar, Deep Fill, 15×100mm plate R	Hardy Diagnostics	Cat # W15
Critical Commercial Assays
Seahorse XF Cell Mitochondria Stress Test Kit	Seahorse Biosciences/Agilent	Cat# 103015-100
MACS Mitochondrial Isolation Kit	Miltenyi Biotec	Cat# 130-096-946
Amplex Red Cholesterol Assay Kit	ThermoFisher Scientific	Cat# A12216
Deposited Data
Experimental Models: Cell Lines
Human Embryonic Kidney Cells (HEK293T)	ATCC	Cat# CRL-3216
Platinum-E (Plat-E) Retroviral Packaging Cell Line	A gift some S. Schwab	N/A
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Jackson Laboratories	Stock No: 000664
Mouse: B6-Ly5.1/Cr Strain ID: B6.SJL- PtprcaPepcb/BoyCrCrl	NCI at Charles River	Stock No: 564
Mouse: Ch25h−/− Strain ID: B6.129S6- Ch25htm1Rus/J	Jackson Laboratories	Stock No: 016263
Mouse: Aim2−/− Strain ID: B6.129P2- Aim2Gt(CSG445)Byg/J	Jackson Laboratories	Stock No: 013144
Mouse: Asc−/− Strain ID: B6.129-Pycardtm1Vmd	Genentech	N/A
Mouse: Nlrp3−/− Strain ID: B6.129S6-Nlrp3tm1Bhk/J	Jackson Laboratories	Stock No: 021302
Mouse: Casp1/11−/− Strain ID: B6N.129S2- Casp1tm1Flv/J	Jackson Laboratories	Stock No: 016621
Mouse: Nr1h3−/− Strain ID: 129-Nr1h3tm1Djm/J	Jackson Laboratories	Stock No: 013762
Mouse: Nr1h2−/− Strain ID: B6.129S-Nr1h2tm1Djm/J	Jackson Laboratories	Stock No: 014635
Mouse: Nlrc4−/− Strain ID: B6N-Nlrc4tm1Vmd	Genentech	N/A
Oligonucleotides
RT-PCR primers for Ch25h Fwd: 5′-GCGACGCTACAAGATCCA-3′ Rev: 5′-CACGAACACCAGGTGCTG-3′	This paper	N/A
RT-PCR primers for Hmgcs1 Fwd: 5′-GGAAGCCTTTGGGGACGTTA-3′ Rev: 5′-ACACTCCAACCCTCTTCCCT-3′	This paper	N/A
RT-PCR primers for Lss Fwd: 5′-GGGCCCTGAATGGAGTAACC-3′ Rev: 5′-GTCCTCGATGTGCAAGCCC-3′	This paper	N/A
RT-PCR primers for Dhcr24 Fwd: 5′-GGACATCATCCCTTTCGGCA-3′ Rev: 5′-TGGGGTAGACGTGGATGTCA-3′	This paper	N/A
RT-PCR primers for Sqle Fwd: 5′-AGTGAACAAACGAGGCGTCC-3′ Rev: 5′-AGCAAGCTTTTCGGAGCTGA-3′	This paper	N/A
RT-PCR primers for Tert nuclear DNA	West et al., 2015	N/A
RT-PCR primers for mtDNA D loop	West et al., 2015	N/A
Recombinant DNA
MSCV-IRES-Thy1.1	Addgene	Plasmid ID: 17442
Software and Algorithms
FlowJo v10	FlowJo, LLC	N/A
Prism 6	GraphPad Software	N/A
Other