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Outline of three different metabolite extraction methods. Three different extractions followed several common steps including amount of tissue, vortexing, ultrasonication, and centrifugation. They are differing only in solvent stage where ammonium acetate methanol (AAM) used 10mM 1:1 ammonium acetate and methanol, phosphate buffer methanol (PM) used 100mM 1:1 sodium phosphate and methanol, and water methanol (WM) used LC/MS grade 1:1 water and methanol.
