Fig. 4.

Hierarchical clustering analysis of three different plant metabolite extraction methods using metaboanalyst 3.0. a) Heatmap generated using sum normalized and auto scaled data based on euclidean distance and ward algorithm. Each column refers to a sample and each row to a metabolic feature across the samples. b) Dendogram derived based on Euclidean distance and ward algorithm as well. Phosphate buffer methanol (PM) extraction method clustered separately than the ammonium acetate methanol (AAM) and water methanol (WM) extraction methods.