Table 2.

List of differentially changing metabolites among the three extraction methods tested. AAM is ammonium acetate methanol, PM is phosphate buffer methanol, and WM is water methanol. Significantly changing metabolites were identified using One-way Analysis of Variance (ANOVA).

Compound name	RT	m/z	AAM extraction	WM extraction	PM extraction	p-value
L-Arginine	0.74	175.12	Up	Up	Down	0.02
Aspartate	0.742	134.04	Up	Up	Down	0.021
L-Histidine	0.71	156.08	Up	Up	Down	0.0001
L-Lysine	0.66	147.11	Up	Up	Down	0.0023
L-Citrulline	1.265	176.103	Up	Up	Down	0.042
L-Valine	0.851	118.086	Up	Up	Down	0.036
Alanine	0.752	90.055	Up	Up	Down	0.035
Uracil	2.914	113.035	Up	Up	Down	0.041
L-Threonine	5.909	120.065	Up	Up	Down	0.012
Pyruvate	1.120	89.024	Up	Down	Down	0.066
Zeatin	6.044	220.118	Up	Up	Down	0.01
Lysyl-Phenylalanine	5.824	294.181	Up	Down	Down	0.051
D-Glucosamine	0.745	180.086	Up	Down	Down	0.063
Propanoate	0.822	75.044	Down	Down	Up	0.045
Lysyl-Threonine	4.288	145.513	Down	Down	Up	0.05
N-nitrosoproline	0.90	145.061	Down	Down	Up	0.022
Tetrahydrouridine	1.077	249.107	Down	Down	Up	0.035
N-acetylornithine	1.025	175.107	Down	Down	Up	0.033
Cytidine	1.844	244.092	Down	Down	Up	0.01
L-Pipecolate	1.355	130.08	Down	Down	Up	0.071