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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Neurochem. 2017 Sep 5;143(5):489–506. doi: 10.1111/jnc.14135

Figure 3. Effects of co-culturing with bEND.3 cells or primary endothelial cells from rat forebrain on GLT-1 and GLAST expression and the effect of co-culture with RBEC on DHK-sensitive Na+-dependent uptake in cortical astrocytes from dual reporter mice.

Figure 3

Astrocytes were cultured directly on top of an intact monolayer of either bEND.3 cells or RBEC for 10–14 days and then harvested for Western blot analysis of eGFP (a), GLT-1 (b), and GLAST (c) protein levels. Top: Representative blots and Bottom: summary of quantification, normalized to β-actin. The β-actin blots are identical in panels (b) and (c), as GLT-1 and GLAST are quantified from the same immunoblot. Data are the mean ± SEM of four independent experiments. ** p ≤ 0.01 for indicated comparison. (d) Glutamate uptake (0.5 μM) was measured after 12–13 days in culture in the absence or presence of 300 μM dihydrokainic acid (DHK). (e) Data from (d) were analyzed to only show DHK-sensitive uptake. Data are the mean + SEM of four independent experiments. * p < 0.05 for indicated comparisons.