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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Neurochem. 2017 Sep 5;143(5):489–506. doi: 10.1111/jnc.14135

Figure 7. Effects of shRBPJ on eGFP and GLT-1 protein levels.

Figure 7

Cortical astrocytes from dual reporter mice were cultured alone or on top of an intact monolayer of bEND.3 cells and infected with an shRNA directed against RBPJ (shRBPJ), a scrambled shRNA control (shScr), or no virus 3–5 days after re-plating. Cell lysates were harvested for Western blot 11–13 days after transduction for analysis of (a) RBPJ, (b) eGFP, and (c) GLT-1 protein levels. Top: Representative blots and Bottom: summary of quantification, normalized to β-actin. Data are the mean ± SEM of six independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 for indicated comparisons.