Skip to main content
. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Parasitol Res. 2017 Aug 21;116(10):2821–2830. doi: 10.1007/s00436-017-5593-9

Figure 1. Expression of rBmHAX and rBmHAT.

Figure 1

The multivalent genes bmhax (1320 bp) and bmhat (957 bp) were amplified and cloned into the expression vector pRSETA and successfully expressed in E. coli BL21 (DE3). Both the expressed proteins were purified by immobilized metal affinity chromatography (IMAC) and purity confirmed by western blot analysis using anti-His antibodies. Purified rBmHAX and rBmHAT were separated on a 12% SDS PAGE gel. Lane 1- Molecular weight marker, Lane 2- rBmHAX (a prominent band at 52 kDa) and Lane 3 - rBmHAT (a prominent band at 39 kDa).