Figure 5.
Effects of cycloheximide on vacuole formation in A549 cells induced by combination PDT (LD90 conditions). Phase contrast (a–d) and ErTr fluorescence (e–h) images were acquired either prior to treatment (a,e) or 16 h after irradiation (b–d, f–h). Treatments were: a,d = untreated controls; b,e = combination PDT; c,f = combination PDT + 0.5 μM cycloheximide added just before irradiation; d,h combination PDT with cycloheximide added 4 h after irradiation.