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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Amino Acids. 2017 Aug 17;49(11):1793–1804. doi: 10.1007/s00726-017-2477-3

Figure 1. NADH-Glo reaction.

Figure 1

Dependence on (A) the concentration of NADH, (B) incubation time and (C) effects of other components. A, NADH (0.5–20 μM) solutions in 0.1 M Gly-NaOH buffers of different pH (8.0, 8.5, 9.0 and 9.5) was reacted with an equal volume of NADH-Glo reagent at RT for 30 min and luminescence was measured in duplicate samples. B. Luminescence measurements of samples in A in pH 9.0 buffer were made after 15, 30, 45 and 60 min of NADH-Glo assay. C. The components of the DHSR and DMSO, were added individually to NADH solutions (5 μM in 0.1 M Gly-NaOH buffer pH 9.0) at the final concentrations indicated and reacted with NADH-Glo reagent. After 30 and 60 min incubation at RT, luminescence was measured. The experiments were performed in duplicate. RLU, relative light unit