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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Amino Acids. 2017 Aug 17;49(11):1793–1804. doi: 10.1007/s00726-017-2477-3

Figure 5. Comparison of DHS partial and complete reaction rates at 37 °C (A, C) and RT (B, D).

Figure 5

DHSR1 was performed at 37 °C or at RT as in Fig. 3A, but using lower levels of the enzyme (0.05, 0.1 and 0.25 μg in 50 μl). NADH-Glo reagent was added after 1 or 2 h incubation of DHSR1. The luminescence (RLU) values (left Y-axis) were converted to pmol of NADH produced per 50 μl reaction mixture (indicated on the right Y-axis). The DHSRC mixture contained the same components as the DHSR1, except that a mixture of radioactive spermidine and cold spermidine was used and the eIF5A precursor was added at 30 μM. The pmol of deoxyhypusine formed per 50 μl DHSRC was estimated as indicated on the Y-axis. The experiment was repeated with similar results and a representative experiment (in duplicate) is shown.