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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Apoptosis. 2017 Dec;22(12):1532–1542. doi: 10.1007/s10495-017-1423-x

Figure 5.

Figure 5

Increased binding of p53 to the extended PUMA promoter in response to culture in DM does not occur in the absence of MyoD binding. For all, equal cell numbers were plated and the next day cultured as indicated. In (A) Chromatin Immunoprecipitation was performed on each cell sample using EZ ChIP™ Chromatin Immunoprecipitation Kit (Upstate) per manufacturer’s instructions using 5 g anti-p53 (OP03-Calbiochem) or IgG control (Sigma-Aldrich). Quantitative PCR was used to assay for the relative levels of the PUMA (−936/−556). Data was normalized to the signal detected from the input of each sample. Error bars represent mean +/− SEM of triplicates. In (B) Western analysis to measure p53 levels was performed as described in Methods. Shown are results from one experiment that are representative of three independent experiments.