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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Mucosal Immunol. 2017 May 17;11(1):50–60. doi: 10.1038/mi.2017.38

Figure 3. Jak32067insC causes the WLVRN phenotype.

Figure 3

(A) Diagram of mouse Jak3 with location of predicted frameshift mutation and introduced stop codon based on WES. (B–H) Analysis of littermates discordant for the WLVRN phenotype, either from (B) original Nr1d1tm1Ven/LazJ pedigree or (C–H) isolated Nr1d1+/+ WLVRN line. (B) Sanger sequencing of Jak3 exon 14 from banked genomic DNA (1A). Representative flow plots of (C) p-STAT5 from CD4+ T cells after 15 minutes of IL-7 stimulation or (D) CD127 expression from resting CD4+ T cell and CD19+ B cells. (E) Immunoblot for Jak3 from BMDMs. (F–H) Total number of ILCs in littermates from (F) Jak32067insC/+ intercross, (G) Jak3tm1Ljb/+ intercross, and (H) Jak32067insC/+ × Jak3tm1Ljb/+ intercross as gated in 1D, but without the use of NK1.1 for Jak3tm1Ljb mice which are on a mixed 129S4 and C57BL6/J background. Data represent (F) n=4 (G) n=5 or (H) n= 6 mice per genotype and (C–D, F–G) 3 (E) 1, or (H) 4 independent experiments. *p<.05, **p<.01, ***p<.001, ****p<.0001 one-way ANOVA with Tukey’s multiple comparisons test.