Table 2.

Details of primers used for amplification and sequencing in this study.

Locus1	Primer Name	Primer sequence (5′→3′)	Annealing temperature (°C)	Orientation	Reference
ITS	V9G	TTA CGT CCC TGC CCT TTG TA	52	Forward	de Hoog & Gerrits van den Ende (1998)
	ITS4	TCC TCC GCT TAT TGA TAT GC	52	Reverse	White et al. (1990)
LSU	LSU1Fd	GRA TCA GGT AGG RAT ACC CG	52	Forward	Crous et al. (2009c)
	LR5	TCC TGA GGG AAA CTT CG	52	Reverse	Vilgalys & Hester (1990)
rpb2	fRPB2-5F	GAY GAY MGW GAT CAY TTY GG	60→58→54	Forward	Liu et al. (1999)
	RPB2-5F2	GGG GWG AYC AGA AGA AGG C	60→58→54	Forward	Sung et al. (2007)
	Rpb2-F1	GGT GTC AGT CAR GTG YTG AA	60→58→54	Forward	Videira et al. (2015a)
	Rpb2-F4	GAY YTB GCI GGI CCI YTI ATG GC	60→58→54	Forward	Videira et al. (2016)
	Rpb2-F5	GCN ACI GGI AAY TGG GG	60→58→54	Forward	This study
	Rpb2-F6	AAR GCI GGT GTI AGY CAR GT	60→58→54	Forward	This study
	fRPB2-7cR	CCC ATR GCT TGY TTR CCC AT	60→58→54	Reverse	Liu et al. (1999)
	Rpb2-R1	TCC TCN GGV GTC ATG ATR ATC AT	60→58→54	Reverse	Videira et al. (2015a)
	Rpb2-R3	ATC ATN GMN GGR TGR ATY TC	60→58→54	Reverse	This study

¹ITS: internal transcribed spacers and intervening 5.8S nrDNA; LSU: large subunit (28S) of the nrRNA gene operon; rpb2 partial RNA polymerase II second largest subunit gene.