Figure 1.

Secretory leukoprotease inhibitor (SLPI) is expressed in basophils and eosinophils but not in mast cells. (A) A quantitative RT-PCR of Slpi in s the bone marrow (BM)-derived basophils, eosinophils, mast cells, and BM cells. (B) Basophils and eosinophils were sorted from the spleen cells after the depletion of CD4⁺CD8⁺B220⁺ cells using magnetic separator. Eosinophils are also sorted from the peritoneal cavity (PEC). A quantitative RT-PCR of Slpi were shown in the indicated cells. (C) Immunoblotting of SLPI in the indicated cells. The data were normalized to the expression of β-actin and presented relative to the expression in BM cells. (D,E) Fluorescence microscopy and transmission electron microscopy (TEM) images of BM-derived basophils (BMBs) (D) and BM-derived eosinophils (BMEos). (E) from B6 and Slpi^−/− mice. Bright field (BF), SLPI (red) DAPI (blue), Diff-Quick staining, alcian blue staining, and TEM images are shown (scale bar: 2 µm). (A,B) Data were normalized to the housekeeping Rps16 (mean ± SD). n = 4. **P < 0.01. (C–E) Data are representative of three independent experiments.