Figure 6.

Secretory leukoprotease inhibitor (SLPI) interact with the JNK-interacting protein 3 (JIP3) scaffold protein, and negatively regulates Toll-like receptor (TLR) 4-mediated Elk-1 activation. (A) B6 and Slpi^−/− bone marrow-derived eosinophils (BMEos) were stimulated with lipopolysaccharide (LPS) (1 µg/ml). Immunoblots of the indicated proteins are shown. The arrows indicate the p54 and P46 isoforms of JNK1. GAPDH was used as loading and internal monitoring controls. (B) The relative intensities of pJNK1/JNK1 and pSer383 Elk-1/Elk-1 in B6 and Slpi^−/− BMEos were estimated by densitometric scanning with normalization to GAPDH (means ± SD). n = 3. *P < 0.05. **P < 0.01. (C) JIP3 and SLPI after the precipitation of anti-JIP3 Ab or control mouse IgG₁ in B6 and Slpi^−/− BMEos. The loading volumes (1/2 and 1/1) are shown. (A,C) Data are representative of three separate experiments.