Skip to main content
. 2017 Nov 13;7:472. doi: 10.3389/fcimb.2017.00472

Figure 2.

Figure 2

The regulatory role of interleukin (IL)-35 in peripheral blood mononuclear cells (PBMCs). PBMCs isolated from patients with chronic hepatitis B (CHB, n = 10) were stimulated recombinant hepatitis B surface antigen (HBsAg) in the presence or absence of recombinant IL-35 for 24 h. (A) Cellular proliferation was measured by cell counting kit-8. The data were presented as mean ± SD, and significances were calculated using paired t-test. (B) Phosphorylated STAT1 (p-STAT1) and total STAT1 were tested by Western blot, and GADPH was shown as control. (C) Representative flow cytometry analysis for apoptotic cells were shown in both PBMCs. Annexin V+PI− cells (lower right) represented early stage apoptotic cells, while Annexin V+PI+ cells (upper right) represented late stage apoptotic cells. The percentages of early stage apoptotic cells (D) and late stage apoptotic cells (E) were shown. The data were presented as mean ± SD, and significances were calculated using paired t-test.