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. 2017 Nov 13;8:1505. doi: 10.3389/fimmu.2017.01505

Figure 1.

Figure 1

Loss of double-positive (DP) thymocytes in the offspring of MRL/lpr mice after prenatal betamethasone treatment. (A) Schematic representation of the MRL/lpr mouse model. (B) Body weight from prenatally betamethasone (Bet) and vehicle-treated (PBS) MRL/lpr mice (n = 42–44 per group, male and female). (C) Total number of thymocytes (n = 10–11 per group, male). (D) Frequency of CD4−CD8− double-negative (DN), CD4+CD8+ DP, and mature CD4+ and CD8+ single-positive (SP) thymocytes (n = 8–10 per group, male). (E) t-SNE representation of the surviving cells in the thymic compartment after prenatal betamethasone treatment according to the expression of CD3, CD4, and CD8. Each dot represents a cell, and the colors show levels of expression of the indicated markers. The plots show one representative animal per group. The rightmost plots display the cell abundance (density) for each population. All analyses were performed at postnatal day 1 (PND1). Unpaired Student’s t-test was used for statistical analysis, **P < 0.01 and ***P < 0.001.