Figure 2.

1-Methyl-dl-tryptophan (1MT) treatment increases the influx of activated dendritic cells (DCs) to the lungs. Number of myeloid (CD11c^high11b⁺), plasmacytoid (CD11c^lowB220⁺), and lymphoid (CD11c^highCD8⁺) DCs obtained 96 h (A) and 2 weeks (B) after infection of 1MT-treated and -untreated A/J and B10.A mice with 1 × 10⁶ viable Paracoccidiodes brasiliensis yeasts. Median fluorescence intensity (MFI) of IA^K, CD86, CD11b, and CD40 expressed by DCs obtained from lungs of A/J and B10.A mice 96 h (C) and 2 weeks (D) after infection. DCs were obtained by fractionation with anti-CD11c magnetic beads and DCs phenotyping determined by flow cytometry. The data represent the mean ± SEM of three independent experiments where the asterisk (*) represents a statistically significant difference between treatments (*p < 0.05, **p < 0.01, and ***p < 0.001) and the hashtag (#) represents the difference between the strains (^#p < 0.05, ^##p < 0.01, and ^###p < 0.001).