Figure 6.

1-Methyl-dl-tryptophan (1MT) increases proliferation of CD4⁺ and CD8⁺ T cells and reduces regulatory T cells (Tregs). Spleen lymphocytes from uninfected A/J and B10.A mice were labeled with CFSE (5 mM) and cocultivated with purified dendritic cells (DCs) obtained from 1MT treated and untreated A/J and B10.A mice infected i.t. with 1 × 10⁶ of Paracoccidiodes brasiliensis yeasts. DCs were obtained at 96 h and 2 weeks postinfection, and cocultivated with CFSE labeled lymphocytes at a ratio of DC:lymphocyte of 1:10. After 3 days, the cells were adjusted to 1 × 10⁶, labeled with specific anti-CD4, -CD8, -CD44, -CD62L, -CD25, and -FoxP3 antibodies. The frequency of naive (CD62L^highCD44^low) and effector/memory (CD62L^lowCD44^high) CD4⁺ and CD8⁺ T cells as well as CD4⁺CD25⁺FoxP3 regulatory T cells (Tregs) was measured by flow cytometry. Values are the mean of three independent experiments. The asterisks represent statistically significant differences between treatments (*p < 0.05, **p < 0.01, and ***p < 0.05). The hashtag marks represent statistically significant differences between strains (^#p < 0.05, ^##p < 0.01, ^###p < 0.001).