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. 2017 Nov 13;8:2127. doi: 10.3389/fmicb.2017.02127

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Copyright © 2017 Vaksmaa, van Alen, Ettwig, Lupotto, Valè, Jetten and Lüke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depth profile of copy numbers of genes of interest obtained by qPCR. In all figures, the soil depth is depicted vertically. Horizontally, the copy numbers obtained by qPCR per gram dry weight are presented in log scale. (A) 16S rRNA gene copy numbers of total archaea amplified with Arch349F/Arch807R primers. (B) 16S rRNA gene copy numbers of Bathyarchaeota amplified with MCG528F/MCG732R primers. (C) mcrA gene copy numbers of Methanoperedens nitroreducens quantified with McrA159F/McrA345R primers. (D) 16S rRNA gene copy numbers of total bacteria quantified with Bac341F/Bac785R primers. (E) 16S rRNA gene copy numbers of NC10 phylum bacteria quantified with p2F_DAMO/p2R_DAMO primers.