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. 2017 Nov 13;8:813. doi: 10.3389/fphar.2017.00813

Table 1.

RT-PCR primers and TaqMan probes.

Genes Primer and probes
hVEGF-Forward TACCTCCACCATGCCAAGTG
hVEGF-Reverse TGATTCTGCCCTCCTCCTTCT
hVEGF-TMProbe* [FAM]TCCCAGGCTGCACCCATGG[BH1]
hGDNF-Forward CGCTGAGCAGTGACTCAAAT
hGDNF-Reverse CGATTCCGCTCTCTTCTAGG
hGDNF-Probe* [FAM]TCCATGACATCATCGAACTGATCAGG[BH1]
hNCAM1-Forward AGATGAGGGCACTTATCGCT
hNCAM1-Reverse GATGGTAGGTGGCACATTCA
hNCAM1-Probe* [FAM]CCGTGCCAGGATTCTGCCCT[BH1]
18S-TM-Forward GCCGCTAGAGGTGAAATTCTTG
18S-TM-Reverse CATTCTTGGCAAATGCTTTCG
18S-TM-Probe* [HEX]ACCGCGCAAGACGGACCAG[BH2]
*

TaqMan probes is labeled at the 5′-end with the fluorescence FAM (HEX) and a 3′ quenching acceptor Black Hole (BH1/BH2).