Table 1.
Genes | Primer and probes |
---|---|
hVEGF-Forward | TACCTCCACCATGCCAAGTG |
hVEGF-Reverse | TGATTCTGCCCTCCTCCTTCT |
hVEGF-TMProbe* | [FAM]TCCCAGGCTGCACCCATGG[BH1] |
hGDNF-Forward | CGCTGAGCAGTGACTCAAAT |
hGDNF-Reverse | CGATTCCGCTCTCTTCTAGG |
hGDNF-Probe* | [FAM]TCCATGACATCATCGAACTGATCAGG[BH1] |
hNCAM1-Forward | AGATGAGGGCACTTATCGCT |
hNCAM1-Reverse | GATGGTAGGTGGCACATTCA |
hNCAM1-Probe* | [FAM]CCGTGCCAGGATTCTGCCCT[BH1] |
18S-TM-Forward | GCCGCTAGAGGTGAAATTCTTG |
18S-TM-Reverse | CATTCTTGGCAAATGCTTTCG |
18S-TM-Probe* | [HEX]ACCGCGCAAGACGGACCAG[BH2] |
TaqMan probes is labeled at the 5′-end with the fluorescence FAM (HEX) and a 3′ quenching acceptor Black Hole (BH1/BH2).