Table 3.
Primary and secondary antibodies used in immunofluorescent staining.
| Antibody | Host | Dilution | Source |
|---|---|---|---|
| ANG | Mouse | 1:150 | Sigma |
| Cx47 | Mouse | 1:150 | Santa Cruz |
| GDNF | Rabbit | 1:100 | Santa Cruz |
| GFAP | Mouse | 1:200 | Santa Cruz |
| GFAP | Rabbit | 1:250 | Santa Cruz |
| HNA (Human Nuclear Antigen) | Mouse | 1:150 | Millipore |
| HSP27 | Rabbit | 1:200 | Abcam |
| NG2 | Mouse | 1:2000 | Abcam |
| Olig2 | Rabbit | 1:150 | Sigma |
| OSP | Rabbit | 1:100 | Santa Cruz |
| PSD95 | Rabbit | 1:200 | Abcam |
| Synaptophysin | Rabbit | 1:150 | Abcam |
| VEGF | Goat | 1:300 | Sigma |
| Vimentin | Mouse | 1:1200 | Santa Cruz |
| Anti-goat IgG conjugated with Alexa 488 | Donkey | 1:200 | Invitrogen |
| Anti-rabbit IgG conjugated with | Donkey | 1:200 | Invitrogen |
| Alexa 555 | |||
| Anti-mouse IgG conjugated with | Donkey | 1:200 | Invitrogen |
| Alexa 647 |