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. 2017 Nov 13;8:911. doi: 10.3389/fphys.2017.00911

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Copyright © 2017 Song, Wang, Li, Chen, Liu, Chen, Li, Li, Li, Lian and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of Leydig cells in the testes after in vivo PTHrP treatment. Immunohistochemical staining of CYP11A1 (A–D) and 11β-HSD1 (E–H) of the testes from the rats treated with 0, 10, and 100 ng/testis PTHrP on post-EDS day 7 for 21 days. (A,E): the control (0 pmol/testis PTHrP); (B,F): 10 ng/testis PTHrP; (C,G): 100 ng/testis PTHrP; (D,H): quantitative data. Blue arrow indicates CYP11A1 positive Leydig cells. Black arrow indicates 11β-HSD1 positive Leydig cells. Bar = 50 μm. Mean ± SEM, n = 6. No significant difference between PTHrP group and the control was observed.