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. 2017 Nov 2;7:13009. doi: 10.1038/s41598-017-12581-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mephedrone in a week monitoring program. Results are displayed as concentrations (columns) in (a), population normalised-loads (columns) in (b) and enantiomeric fractions (symbols). The unpaired t-test showed “t Stat > t Critical one-tail” for all wastewater samples excluding Wednesday in 2014 (8.80 > 4.78), p one-tail (0.000024) < 0.001 and for all wastewater samples excluding Monday in 2015 (2.83 > 2.01), p one-tail (0.018) < 0.05. Therefore, EFs from wastewater samples were significantly different (EF > 0.5) from EF = 0.5 during validation. Paired t-test results showed “t Stat < t Critical one-tail” (1.14 < 1.94), p one-tail (0.15) > 0.05, so two datasets of wastewater samples were not significantly different from each other.