Table 1.

Overview of mephedrone and its metabolites through target and non-target screening in all the samples investigated in this study (only target screening was performed in pooled urine).

Analyte	Street mephedrone	Rat urine	Pooled urine	pHLM	Wastewater
Mephedrone	X	X	X	Xb, Xc	X
Normephedrone		X		Xb, Xc
1-dihydro-mephedrone				Xc	X
1-dihydro-normephedrone		X			X
4′-hydroxy-mephedrone		X		Xb	X
4′-hydroxy-normephedronea					X
1-dihydro-4′-oxomethyl-normephedroned		X
4′-carboxy-mephedrone		X		Xb	X
4′-carboxy-normephedronee		X		Xc	X
Hydroxy-mephedrone-3O-glucuronide		X
Hydroxy-normephedrone-3O-glucuronide		X
Normephedrone-N-glucuronided				Xc
Normephedrone-N-sulphated					X

Legend: ^ametabolites mentioned in¹⁴, ^bthose formed in pHLM incubation through set A experiment and ^cthrough set B,^dthose not previously published, ^ethose mentioned in³⁴.