Fig. 3.

Disruption of retinal structure and function after Dicer1 deletion in MG in either juvenile or adult mice. a Immunofluorescence for tdTomato (endogenous, unlabeled), Otx2, and DAPI of retinal cross sections of an adult Dicer-CKO_MG mouse 12 months after Dicer1 deletion, which received Tamoxifen at postnatal day (P) 11–14. b, c Number of Otx2⁺ cell layers in the ONL in the central (b) and peripheral retina (c) 1, 6, and 12 months after Dicer1 deletion in the wild-type (wt_{1 mo} n = 3, wt_{6 mo} n = 4, wt_{12 mo} n = 3) and Dicer-CKO_MG (CKO_{1 mo} n = 4, CKO_{6 mo} n = 4, CKO_{12 mo} n = 7). d OptoMotry results for wt (n _{2 weeks} = 4, n _{3 weeks} = 3, n _{5 weeks} = 4, n _{12 weeks} = 3, n _{15 weeks} = 3, and n _{26 weeks} = 3, one measurement per time point) and Dicer-CKO_MG mice (n _{2 weeks} = 4, n _{3 weeks} = 4, n _{5 weeks} = 5, n _{12 weeks} = 3, n _{15 weeks} = 3, and n _{26 weeks} n = 3, two measurements per time point), 2–28 weeks after Dicer1 deletion (p _{2 weeks} = 0.000, p _{3 weeks} = 0.001, p _{5 weeks} = 0.002, p _{12 weeks} = 0.004, p _{15 weeks} = 0.007, p _{26 weeks} = 0.000). e Experimental design for Dicer1 deletion in the adult mouse. f–i Immunofluorescence for tdTomato (endogenous unlabeled) and DAPI nuclear labeling of Dicer-CKO_MG mice in which Dicer1 was deleted at postnatal day (P) 56–59 (2 months of age), 2 months after deletion (f, g) or 5 months after deletion (i). h Number of tdTomato⁺ MG in the INL in wt (n = 3) and Dicer-CKO_MG mice (n = 3) 2 months after Dicer1 deletion. Statistics: mean ± S.D., Shapiro–Wilk test for normality, for b, c: Mann–Whitney U-test, for d, h: Levene’s test for equality of variances and independent samples t-test (2-tailed), significant differences are indicated, *p < 0.05, **p < 0.01, ***p < 0.0001. Mice of at least two different litters were analyzed. Scale bars 100 µm. OPL, INL, IPL, GCL as defined in Fig. 1 legend