Fig. 6.

miR-9 targets Bcan and regulates Müller glial morphology. a Schematic of the Bcan 3′ UTR-GFP sensor. b–g Live images of 3T3 cells fibroblasts transfected with the Bcan 3′UTR sensor (plasmid) and mCherry mRNA transfection control with either control mimics or mimics for miR-9 or let-7a. h Quantification of the number of Bcan 3′UTR-GFP sensor⁺ 3T3 cells of the total number of transfected cells (n _ctl = 5, n _miR-9 = 5, n _miR-124 = 4, n _let-7a = 4 n _miR-181a = 2, and n _miR-125b-5p = 2 individual experiments for two wells per condition per experiment). i Cell culture and transfection (TF) scheme. j–l Live images of wt MG (j) and Dicer-CKO_MG cultures (k, l) treated with control or miR-9 mimics. m Quantification of the aggregation index of wt, and Dicer-CKO_MG either transfected with control miR or miR-9, n = 3 mice for each condition. This experiment was performed four times in the laboratory. n Experimental design explant cultures. o–q Immunofluorescent labeling with antibodies against tdTomato and DAPI nuclear staining of P16 wt (o), Dicer-CKO_MG with control mimics (p), and Dicer-CKO_MG explant cultures treated with miR-9 mimics (q), 7 days ex vivo (DEV). r Numbers of tdTomato⁺ MG per field in the ONL/OPL in wt (n = 3 mice) and Dicer-CKO_MG + control miR (n = 3 mice) and Dicer-CKO_MG explants treated with miR-9 mimics (n = 3 mice). This experiment was performed twice in the laboratory. Statistics: mean ± S.D., Shapiro–Wilk test for normality, for h, r: Mann–Whitney U-test, for m: Levene’s test for equality of variances and independent samples t-test (2-tailed) as well as Bonferroni–Holm correction for all tests, significant differences are indicated, *p < 0.05, **p < 0.01, ***p < 0.0001. Scale bars 100 μm. dpTF, days post transfection; ONL, INL, GCL as defined in Fig. 1 legend