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. 2017 Nov 17;7:15767. doi: 10.1038/s41598-017-16116-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Labeling of Chlamydia-infected cells with NBD-PS. HeLa cells were grown on coverslips and infected with C. trachomatis strain D. After 24 hours, 1 µM NBD-PS (green) was added to the medium. The cells were washed and fixed after 1 hour of incubation. DNA was stained with Hoechst dye (blue), and imaging was performed with a Keyence microscope equipped with a 40x objective. Panel B shows a magnified cropped image of an infected cell with 2 inclusions, which are indicated. Images were taken from a single labeling experiment.