Fig. 1.

TDF/FTC/RAL combined medication affects NSC homeostasis and progenitor proliferation in the mouse DG. 10-week-old C57BL/6 mice were injected daily with either TDF/FTC/RAL (104/120/28 mg/kg) or vehicle control for 60 days. a Experimental design for long-term BrdU labeling experiments. BrdU was injected along with the drugs at 5 μl/g for 5 consecutive days from the first day of drug treatment. b-g Representative confocal microscope images of coronal sections through the mouse hippocampal DG immunolabeled for DAPI (blue) and BrdU (green). Bottom right corner of each image shows the BrdU-positive cells in the molecular layer of the DG. h Experimental design for short-term BrdU labeling experiments. BrdU was injected at 10 μl/g 2 h before euthanization on the day following the last drug treatment. i-n Representative confocal microscope images of coronal sections through the mouse hippocampal DG immunolabeled for DAPI (blue) and BrdU (green). Bottom right corner of each image shows the BrdU-positive cells in the molecular layer of the DG. Quantification of total BrdU-positive cells was shown in o and p. * denotes p < 0.05. Scale bar: 200 μm. n = 6 for each group