Fig. 2.

TDF/FTC/RAL combined medication reduces viability and proliferation of mouse NPCs in vitro. Mouse NPCs were treated with either DMSO a or doses of TDF/FTC/RAL b-d. After 48 h, cells were fixed and stained with DAPI (blue), anti-Ki67 (green), and anti-Nestin (red) antibodies. Quantification of total cell number was shown in e. Quantification of the ratio between Ki67-positive and DAPI-positive cells was shown in f. ** denotes p < 0.01, *** denotes p < 0.001. Scale bar: 100 μm