Table 1.

Contingency analyses of coding region substitutions in the Hawaiian and North American genes

	ASAP3/TM6		ASAP1		ASCAB9
	HI	NA	HI	NA	HI	NA
Nonsynonymous	25	5	28	50	7	3
Synonymous	15	31	11	64	9	31
	P = 0.00001**		P = 0.002**		P = 0.007**

The numbers of nonsynonymous and synonymous nucleotide substitutions in the coding regions were inferred along branches of the gene phylogenies using maximum likelihood ancestral state reconstructions (see Fig. 3). Inferred substitutions along the phylogenetic branches at the bases of the A and B copies of the ASAP3/TM6 and ASAP1 loci in the Hawaiian species (denoted in black in Fig. 3) were not included in the analyses, as the substitutions may have occurred in genes of either North American or Hawaiian species. HI, Hawaiian; NA, North American. Significance was calculated by using Fisher's Exact Test (**, P < 0.01; 

^**, P < 0.001).