Fig. 2.

PTK6 downregulation suppresses growth and induces death of ER+ breast cancer cells. a MCF-7 or T47D cells or b MCF-7L-TamR or T47D-TamR infected with control shRNA (EV) virus or either of two PTK6 shRNA (C9 and 49) viruses were seeded onto chamber slides coated with 3D Matrigel^TM. Cells were re-fed with complete media every 3 days and growth was monitored. Scale bars represent 30 μM. Lysates from the PTK6 shRNA infected cells (MCF-7 at 96 h or T47D at 120 h post infection) were probed with the indicated antibodies. All experiments were performed three times. All the western blot images were processed in parallel. c MCF-7 or MCF-7LTamR cells expressing control or PTK6 shRNAs (C9 or 49) were injected subcutaneously into the right flank of 6-week-old female nude mice implanted with 17β-estradiol pellet (n = 5/group). Tumor size was measured every 3–4 days