Fig. 3.

PTK6 downregulation suppresses growth of endocrine therapy-sensitive and resistant ER+ breast cancer cells. MCF-7-EDR, T47D-EDR, or MCF-7-FulvR cells infected with control shRNA (EV) virus or either of two PTK6 shRNA (C9 and 49) viruses were seeded in monolayer cultures (a) or 3D Matrigel^TM cultures (b). Cells were re-fed with fresh growth media every 3–4 days. The cells in the 3D cultures were visualized on day 10. Lysates from the PTK6 shRNA-infected cells were probed with the indicated antibodies. Scale bar: 30 μm. All experiments were performed three times. All the western blot images were processed in parallel