Fig. 4.

PTK6 downregulation induces apoptosis of endocrine therapy-sensitive and resistant ER+ breast cancer cells. a Cells expressing control or PTK6 shRNAs (C9 or 49) were lysed at 96 h (MCF-7, MCF-7L-TamR, MCF-7-EDR, MCF7-FulvR) or 120 h (T47D, T47D-TamR) following infection with shRNA lentivirus. Lysates were probed with antibodies to cleaved PARP, PTK6, and GAPDH. Experiments were performed three times. All the western blot images were processed in parallel. b MCF-7L-TamR or T47D-TamR cells expressing control or PTK6 shRNAs (C9 or 49) were stained with AnnexinV and PI, and analyzed by flow cytometry. Experiments were performed five times