Figure 2.

Frequency of substitutions (A) and degree of variability (B) of residues encoded by transcripts in bone marrow (BM) with an origin in the 11 investigated germline genes. Residues 27–38 code for complementarity determining region (CDR) 1, while residues 56–65 code for CDR2. Variability was calculated as (the number of amino acids encoded by more than 1% of all reads)/(fraction of reads encoding the most common residue). Only sequences showing no evidence of insertions and deletions were included in the analysis. Numerous residues differed substantially between germline genes in the degree of targeting by substitution. Note the substantial degree of substitution also in some residues of framework region (FR). Frequency of substitution and variability for individual genes are shown in Figure S2 in Supplementary Material.