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. Author manuscript; available in PMC: 2017 Nov 18.
Published in final edited form as: Curr Biol. 2016 Aug 18;26(18):2446–2455. doi: 10.1016/j.cub.2016.07.048

Figure 1. Single-cell RNA-seq of ALA, the neuron central to C. elegans stress-induced sleep.

Figure 1

(A) Stress-induced sleep is regulated by LIN-3C (EGF) and LET-23 (EGFR) expressed on the surface of ALA. In this work, we study the mechanism of sleep induction downstream of ALA. (B) Single-cell RNA-seq expression data of 8,133 protein-coding genes (grey) collected from two pools of microdissected ALA neurons (four and five cells; see also Figure S1; Table S1) compared with mixed-stage whole larvae. The ratio of expression level of protein-coding genes from the ALA neuron versus whole larvae shows that four neuropeptide-coding genes have ≥10-fold higher expression in ALA than in whole larvae: flp-24, flp-7, flp-13, and nlp-8 (highlighted with colored squares for flps, and a green triangle for nlp-8; see also Figure S2). Expression levels of other flp and nlp coding genes are also highlighted by red squares and blue triangles respectively (see also Table S2). RPKM unit: reads per kilobase of transcript per million mapped reads.