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. Author manuscript; available in PMC: 2017 Nov 18.
Published in final edited form as: Curr Biol. 2016 Aug 18;26(18):2446–2455. doi: 10.1016/j.cub.2016.07.048

Figure 5. Overexpression of NLP-8 inhibits defecation while pumping continues.

Figure 5

(A) Average number of defecation events for individuals five minutes before (PRE) and one hour after (POST) heat shock. Overexpressing flp-13 or nlp-8 inhibited defecation. (B) Pumping rate was scored from 10-second video recordings before and one hour after heat shock. (C) Overexpression of either nlp-8 or flp-13, but neither flp-24 nor flp-7, inhibited defecation. The thick line indicates strong and independent inhibition of defecation, while the thinner line indicates inhibition of defecation that may be a consequence of pumping quiescence. (A) & (B) Data shown as mean±SEM; n≥10 C. elegans for each strain (see also Figure S5A); ***p<0.001; paired t-test.