Figure 3.

p45 NF-E2 and MafK recruitment during induction. Anti-p45 NF-E2 antibody (A and B) and anti-MafK antibody (C and D) were used for ChIP experiments. Duplex PCR was performed by using the cross-linked chromatin from uninduced (A and C) and induced 745A MEL cells (B and D). ChIP and PCR reactions were repeated at least twice to verify results. Quantification of duplex PCR results is shown. The ratio of products obtained with β-globin gene locus (Globin) and myoD1 gene (M) primers was measured as described in Fig. 2.