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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Bone. 2017 Oct 12;106:103–111. doi: 10.1016/j.bone.2017.10.012

Figure 1.

Figure 1

A–H: Gene expression profiling from cells of the NF1 PA site. Bulk: mixed cells of the NF1 PA site; Clonal −/−: Single cells from the NF1 PA site; Clonal +/−: single cells from the iliac crest cultures from the same patient. I: Nf1 expression in WT (Nf1f/f +Ad-GFP) and Nf1-deficient (KO, Nf1f/f +Ad-CRE) mBMSCs (qPCR, n=3). J–L: Tgfb1 expression in murine WT and Nf1-deficient mBMSCs (J), MEF cells (K) and calvaria cells (L)(qPCR, n=3). M–O: TGFβ1 protein expression in WT and Nf1 KO BMSCs using ELISA (M, n=3) and Western blotting (N, n=3). O–P: Measurement of TGFβ-1/SMAD signaling activity in the conditioned medium collected from cultures of WT and -deficient mBMSCs (n=3) using Luciferase assay (O, n=3) and p-SMAd2 level (P, n=3, TGFβ1 positive control: 5ng/ml). n.s: non-significant, *: p < 0.05 between genotypes, qPCR gene expression is normalized by Hprt expression.