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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Immunol. 2017 Oct 20;92:106–115. doi: 10.1016/j.molimm.2017.10.015

Figure 1. PCR and western immunoblotting analyses of P. aeruginosa strains.

Figure 1

(A) Diagnostic PCR amplification of waaL deletion (waaL) in the wt and motABmotCD backgrounds of the PA14 and PAK strains of P. aeruginosa. Using the primers described in the materials and methods, deletion of the native locus was tested with a PCR reaction containing a reverse primer within the waaL coding sequence (top panel) and one using flanking sequences that shows the decrease in size from the deletion of the waaL coding sequence. (B) LPS from wt PA14 and PA14 waaL was prepared by proteinase-K digestion and probed with rabbit polyclonal antisera raised against the serotype O-19 strain.